Part:BBa_K112006:Design

T4 antiholin! in BBb

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 206
  

Design Notes

N/A

Source

PCR bx7/bx8 on Bjh1303		(325 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112006
----------------------------------------
bx7		Forward biobricking of {T4 Antiholin!}
ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac
bx8		Reverse biobricking of {T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc